I have been using a RNA clean up kit to improve my A280/260 and A260/30 ratios but the I lost so much RNA, the A260/280 improve and the A60/230 was lower.
Sample Before After
Conc ng/ul 460 55.6
A260/280 1.39 1.98
A260/230 0.84 0.27
Abhijit Maji
It is 8 times lost of your RNA but quality of RNA was improved a lot, you can do two things- first if your requirement is low then proceed with the above protocol secondly if you want a high quantity then just treat your RNA samples with DNase , check on the gel (quality) and use directly for downstream processes.
0 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Biomolecules
- Nucleic Acids
- RNA
More Ashley Riera Blanco's questions See All
Similar questions and discussions