Hi. I am working with placental explants and I want to measure explant viability but using a more faster and cheaper method instead of LDH activity in culture media. I know that MTT and TTC methods are too invasive to work later with explant tissue that's why most people use LDH assays.
I am wondering if other kind o vital staining such as fluorescent staining, FDA/PI, DAPI, etc, will interfere later to extract the mRNA to perform gene expression analysis by qPCR. Are these kind of fluorescent viability assessments possible in explant tissues?