Some white growth in the phosphate buffer

01 January 1970 1 261 Report

I noticed some white growth in the phosphate buffer that was prepared just a week ago. What could be the cause of this, and how can it be resolved?

The buffer wasn't filtered.. Could this be the cause?

Can we autoclave phosphate buffer to resolve this issue?

Sekar Thangaraj

Phosphate buffer got white precipite when it kept at low temperature. It is not a contaminant growth.

Allow the buffer to reach room temperature and dissolve the precipitate or use the water bath.

It's better to prepare 10x stock solution and make it to 1x whenever need.

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