Shotgun in proteomics grant a global protein identification. Normally, all proteins separated on SDS-PAGE should be included in the identified proteins in shotgun, since the latter covers the whole proteome of a sample. I found some proteins on SDS-PAGE that were not present in the identified proteins from shotgun...
Asmae Laouina
Dear Sebastian Schmitt
,Thank you for your answer. The whole proteome results was about 600 proteins. What I understood from your commentary is that some proteins might be missing in the whole proteome due to their low abundance ?
if that's so, it means that I might detect them if I use a different gradient-length in the MS identification ?
kind regards
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