Asmae Laouina

4 Questions 3 Answers 0 Followers

Questions related from Asmae Laouina

Sharp/bright fluorescent images vs pale?

Hello, I have troubles to understand why some fluorescent images look sharp and bright (picture on the left) while others look pale (picture on the right). They were imaged with different...

11 April 2023 5,295 0 View

DAPI staining in multiplex IHC is fading with stripping rounds?

Hello, I currently do multiplex immunostaining on mouse small intestine swissroll sections. We apply the protocol described by Adrien et Guillot al (Deciphering the Immune Microenvironment on A...

29 March 2023 4,654 2 View

How does Multiplex Immunostaining work?

Multiplex IHC is based on multiple rounds of incubating/detaching antibodies (+6 ABs) to assess expression of several proteins on the same sample. However, I didn´t find so far an explanation of...

02 November 2022 2,521 2 View

Some proteins identified from an in-gel digest of some bands in SDS-PAGE are not found in the whol cell shotgun. What could be the reason ?

Shotgun in proteomics grant a global protein identification. Normally, all proteins separated on SDS-PAGE should be included in the identified proteins in shotgun, since the latter covers the...

16 October 2019 9,863 1 View