Dear Prabhash,

Following are the most used tools, you can use couple of them and compare output.

https://eu.idtdna.com/site/order/designtool/index/DSIRNA_CUSTOM

https://rnaidesigner.thermofisher.com/rnaiexpress/

http://dharmacon.gelifesciences.com/design-center/

http://www.protocol-online.org/prot/Research_Tools/Online_Tools/SiRNA_Design/index.html

Thanks

CP Singh

Thank you Chandra. I will surely use the suggested softwares.

Dear Prabhash,

Try Primer3 online tool:

http://bioinfo.ut.ee/primer3-0.4.0/

We used it with success in or study on RNAi in S. mansoni on multitude of gene transcripts.

Best wishes,

Sasa

Thank you for sharing the information Sasa.