I have performed toxicity studies of plant extracts (ethanolic) and no signs of toxicity are observed. I need to complete an experiment on three groups and I am confused about what dose to prepare for the experiment.
You can start with a pre-experiment to determine the appropriate dose range. You can start with 0,5,10,15% with simply 1 or 2 replicates and see what happens. If no mortality is seen, employ greater concentrations such as 0,5,10,15,20,25%, and so on. It would be best to keep going until you find 100% mortality. If you acquire 100% mortality with a concentration of 20%, you can choose 0, 5, 10, and 20% concentrations with mortalities ranging from 0-100% for your real experiment. I hope this will help you.
@ Prabhat Kumar, this depend on the method you're adopted to do the toxicity test either lorkes or OECD guideline, if @ 5000 no toxicity was seen you give percentages of the 5000mg/kg body weight that will represent high , low and medium dose, or alternatively give 3 percentages of that represent high, low and medium of the mean lethal dose.
Hi Prabhat! I'm assuming you have seen no toxicity with your extracts up to at least 2000 mg/kg. I would suggest you do the following if your plant/extract has been used in any traditional system of medicine for any indication (this is very likely) 1)Identify the human dose 2) Calculate the animal equivalent dose using the appropriate conversion factor for rats/mice. 3) Since you need three doses consider that as the median dose and the other two doses as half and double respectively. This may increase your chances of seeing efficacy in your animal studies. All the Best!
(Nair AB, Jacob S. A simple practice guide for dose
conversion between animals and human. J Basic Clin Pharma 2016;7:27-31.)
- Badges
- Science method