My tyloxapol-treated mice (300 mg/kg) show an increase in SOD activity. Can someone give a reference for it
Geology Doctorate. Available for Post-doc and guest faculty. Please give suggestions. Anywhere in world.
09 June 2024 1,805 4 View
CFA - Complete freund's adjuvant
11 March 2024 6,333 0 View
How to count neutrophil
26 February 2024 5,068 3 View
Urban bias in India’s public distribution system
31 December 2023 8,639 1 View
I have bone marrow derived GM-CSF differentiated DCs pulsed with ova & stimulated with TLR ligand. Used these DCs to co-culture OT1 T-cells. After 3 days when I check T cell proliferation by...
19 December 2023 7,356 4 View
I used same dilution for negative as well as positive control
27 June 2023 9,647 0 View
My company is looking to design a L&D program for Employee Skills Identification. We are looking to use BCI technology for the same and it would be of great help if we can find an SME with...
21 June 2023 4,987 1 View
I have performed toxicity studies of plant extracts (ethanolic) and no signs of toxicity are observed. I need to complete an experiment on three groups and I am confused about what dose to prepare...
27 April 2023 7,233 3 View
my plant extract shows less antioxidant activity (by DPPH assay) in medium doses. what could be the reason for it?
17 April 2023 8,527 3 View
There are many kits available which indicates the mitobiogenesis level based on COX1 to SDHA ratio. But I cannot understand the reason behind it. Why not to use SDHA/COX1 ratio as both of the...
12 March 2023 2,375 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,708 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,483 5 View