my plant extract shows less antioxidant activity (by DPPH assay) in medium doses. what could be the reason for it?
What method of extraction did you use? The less DPPH activity observed maybe from extraction process. Regards
Dear Yasha Jha,
Reaction mixture containing different concentrations of HAE (hydroalcoholic extract) of extract (25, 50, 75 & 100 µg/mL) was added to a DPPH reaction mixture. The reaction mixture was shaken overwhelmingly and kept in the dark condition at room temperature for 30 minutes. The absorbance of the reaction mixture was measured at 517 nm spectrophotometrically. The absorbance of every reaction mixture was recorded in triplicate. The scavenging capabilities of DPPH radical were given in the percent inhibition formula using the following equation.
% inhibition= [1- (As– Ab)/Ac] × 100
Where, Ab represents blank absorbance
As stands for the sample absorbance
Ac is the control absorbance
Results were given in IC50 value, a concentration at which half of the radical’s generation was scavenged by the respective plant extract obtained from the percent inhibition value of samples.
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