Single Primer PCR - FAQS.TIPS

04 May 2011 1 4K Report

Hello everyone,

Has anyone successfully performed single strand PCR for sequencing? In essence, I have extracted a genome from a stable transfectant I created. But I'm not sure where the gene integrated. I imagine I could do single stranded PCR priming for the gene that I integrated and find the flanking regions from the genome, but I am not sure. If anyone could help me or direct me to a good protocol I would really appreciate it.

Thank you,

Daniel Balazs

Kaushik Bhattacharjee

You can refer "Direct sequencing by thermal asymmetric PCR (doi: 10.1093/nar/19.17.4783)"

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