What is your sample? You can use RIPA lysis buffer for extraction, and then proceed with western blot protocol

https://www.hindawi.com/journals/mi/2019/7479107/

There are IL-1B antibodies available, you can use that for your western blot.

https://www.abcam.com/il-1-beta-antibody-ab2105.html

You can also use commercially available IL-1B ELISA kit

https://www.perkinelmer.com/product/alphalisa-il1-beta-kit-500-assay-pts-al220c

https://www.bosterbio.com/human-il-1-beta-picokine-trade-elisa-kit-ek0392-boster.html

Just for reference https://www.jimmunol.org/content/jimmunol/early/2017/09/13/jimmunol.1700245.full.pdf?with-ds=yes

Hello Bhairavi, my sample is in media supernatant. do i need to concentrate before adding ripa?

no need to ADD lysis buffer to conditioned media. Concentration may help. you should also be able to see it in the lysate prior to cleavage and release by Caspase 1. Remember some cells do not express caspase 1 and the IL-1 is retained in the cells.

got you Tory! Thanks Tory and Harshul!!