Application for Ph.D. in Inorganic Chemistry?

I hope to have the opportunity to continue toI hope to have the opportunity to continue to do more in-depth research, do more in-depth research. My main field is to use semiconductor metal oxides...

04 January 2021 1,148 2 View

Why oxidation with peracetic acid engender staining of oxytalan fibers with clastic tissue stains?

Just a quick question above, this oxidation method is mentioned by literature, but no one talked about the principle. Can anyone figure out how the oxidation works on any specific group?

20 December 2020 5,878 1 View

Why does my HPLC filter always generate air bubbles?

I have an old HPLC machine. Every time when I put my filter into 50%:50% water and acetonitrile mixture, my filter always generate bubbles. However, once I change the solvent to 100% acetonitrile,...

08 March 2020 5,587 4 View

What are those clay/silt aggregates? Are those intraclastic clast or flocs?

These deposits were from mud caps of sediment gravity flow deposits. These mud caps are homogenous and no trace fossils can be identified. Can any one help me identify these aggregates? Thank you...

10 September 2018 3,627 3 View

How to measure cell density of bacteria entrapped in hydrogel?

How to determine the cell density or total suspended solids of the bacteria which are entrapped in hydrogel like PVA+Alginate?

26 March 2018 436 3 View

How to compute the band structure of TM mode for 2D photonic crystals by COMSOL?

Can we use the COMSOL to compute the band structure of TM polarization for the 2D photonic crystals? If yes, how to do it? With the "Bandgap Analysis of a Photonic Crystal" in application gallery,...

19 September 2017 1,620 1 View

Why do the same primer real time PCR amplification efficiency is not the same every time?

Why do the same primer real time PCR amplification efficiency is not the same every time?

18 September 2017 8,644 2 View

Flame Retardant materials

I am looking to find commercial available Flame retardant materials so that I can implement them in my plastic pallets which are HDPE-silica composite. The presence of silica do enhance the FR...

09 July 2017 2,900 1 View

MiRNA cDNA synthesis kits

I am looking to run some qRTs on some isolated miRNA. I've used a kit to isolate the small RNAs from the total RNA. Is there a need for me to get a cDNA synthesis specific for miRNAs? If so, why?...

18 May 2017 3,552 4 View

"Inflation and the Measurement Problem" written by S. Alexander, D. Jyoti, J. Magueijo. Why their model solve the cosmological measurement problem?

02 May 2017 9,389 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View