Hi, could someone please help me with gene knockout in Klebsiella (homologus recombination) and also I need suggestion for choosing the plasmid?

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Michael J. Benedik

You can use the same methods for gene knockouts in Klebsiella that have been described for E. coli. Plasmids and such from E. coli should replicate and function well in Klebsiella.

Shan Sts

Thank you !!

If anyone can guide me on how to design the primer and antibiotic gene cassette would be of great help because Im so new to this work.