Why oxidation with peracetic acid engender staining of oxytalan fibers with clastic tissue stains?

Just a quick question above, this oxidation method is mentioned by literature, but no one talked about the principle. Can anyone figure out how the oxidation works on any specific group?

20 December 2020 5,878 1 View

What modeling software can be used to analyse fate of pesticides using a fugacity multimedia approach?

Hello, I am trying to understand the fate of pesticides based on the fugacity multimedia approach. Is there software for such modelling? I know SWAT can be used for watershed-level pesticide...

15 October 2020 1,424 3 View

How to amplify extremely high GC-rich template?

I am cloning a whole cDNA of a particular gene and I have designed forward primer at start of 1st exon. The region downstream of forward primer is extremely high GC rich (around 85-89%). Because...

17 September 2020 6,571 11 View

How to apply load in abaqus ?

I have created a beam and i dont know, what is the maximum load it can take, Is there any way to increase the load continuously without giving a fixed value, so that at some load the beam will...

02 September 2020 6,246 9 View

I have a list of properties of tool steels here. Please let me know which of the below mentioned properties will change after heat treatment?

16 August 2020 730 8 View

How to define hyper elastic UMAT in a UEL?

Hi all, I am working on Phase-Field UEL for hyperelastic materials. I am using codes of Dr Molnar ( http://www.molnar-research.com/tutorials_videos_3.html) to develop hyperelastic material. I...

07 April 2020 4,243 1 View

Why does my HPLC filter always generate air bubbles?

I have an old HPLC machine. Every time when I put my filter into 50%:50% water and acetonitrile mixture, my filter always generate bubbles. However, once I change the solvent to 100% acetonitrile,...

08 March 2020 5,587 4 View

Related to Jasco FP-8300 spectrofluorometer?

I used Jasco FP-8300 spectrofluorometer to take a spectra of riboflavin but I got two instead of one. I had changed different solvent but the spectra remained same. Is this instrument fault or...

15 September 2019 633 1 View

What's going wrong with plastic sectioning?

I am doing Aniline blue staining in rice anthers to detect callose signaling. When anthers are squeezed and stained, I could detect the signaling. However, when I do plastic embedding with...

10 April 2019 9,131 15 View

What would be the formula for finding the stretch force for expanding an O ring made of elastic ? On what factors the expansion stretch force depends?

I want to expand an O ring made of elastic material and am looking for the mathematical expression (dynamics) on which the stretch force depends. In addition to this, how does it vary in different...

18 March 2019 4,158 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Is it possible to obtain a negative cronbach alpha?

Good evening everyone. If a questionnaire has 4 factors measuring different constructs as for example CAMI questionnaire measuring attitudes toward the mentally ill that has four factors:...

18 February 2021 3,223 3 View

XRPD Quantification - Building a calibration curve of drug excipient?

I am aiming to utilise XRPD to quantify a drug excipient within a drug product and have been having a little trouble building the calibration curve to allow for this quantification up until this...

10 February 2021 6,145 3 View

Can I use video interview excerpts from others in public domain (YouTube, Vimeo, etc) for my empirical analysis?

Dear community, I am currently writing my master's dissertation. As a part of the method, I have to include views (interview) of experts for the empirical analysis. The problem is I have not...

31 January 2021 3,438 3 View

Having the moderator continuous or dichotomous? Using MANOVA or the Process macro (Hayes)?

Hi community! Currently finalising my Master thesis research. I manipulated my independent variable to have three conditions. Originally, I proposed a 3(3 conditions of independent v)x2...

30 January 2021 7,821 6 View

What is the best way to differentiate L. rhamnosus and L. casei in plate co-cultivation?

I'm having troubles in the differentiation and quantitation of each bacteria population during co-cultivation. The morphologic characteristics are very similar and it's really difficult to count...

13 January 2021 1,550 1 View

How can I selectively repress glucocorticoid receptor translocation to the mitochondria in primary cell lines without altering nuclear GR signaling?

How can I selectively repress glucocorticoid receptor translocation to the mitochondria in primary cell lines without altering nuclear GR signaling? Any thoughts on applicable small molecules to...

06 January 2021 4,324 2 View

How the year 2021 can be made good for global community?

Many steps can be taken to make the year 2021 good for humanity. For instance, if powerful and rich countries decide and spend some part of their resources for the improvement and betterment of...

30 December 2020 830 4 View

What was the most worst thing and what was the most beneficial thing that happened for global community in 2020?

Many bad and good things happened for global community in 2020. However, the present question is about one top most worst thing and one top most good thing that happened in the world in 2020 and...

30 December 2020 6,262 3 View