As the RBC are nucleated i should take the supernatant afer RBC lysis buffer treatmen is it right?
Also the centrifugation speed was kept at 3000rpm for 10 mins at 4 degrees is it right?
Assessing the Feasibility of Vacation Homes and Vehicle Rentals in India In my article, "Assessing the Feasibility of Vacation Homes and Vehicle Rentals in India: Understanding Market Needs,...
03 July 2024 5,535 1 View
How the electron and hole pockets near the fermi level in the band structure affect the thermoelectric properties of the topological half-Heusler compound?
26 June 2024 2,933 0 View
why uv is done and why photocatalytic measurement is done although both use uv radiation
09 June 2024 8,750 1 View
this electrode can't be open.
13 May 2024 2,991 3 View
which ASTM D USE for lap shear strength in UTM of wood substrate
04 April 2024 4,791 0 View
Hey, I am doing an research on how parenting styles create negative attachment styles in young adults. I can't seem to understand what's the criteria for assessing and how to use it. It would be...
02 April 2024 7,017 2 View
Where did i got my research gate id?
21 March 2024 5,116 0 View
Please suggest journals related to plant science or analytical plant chemistry that are indexed in Scopus. The journals must be free to publish in, regardless of their impact factor.
04 March 2024 1,393 2 View
i am doing Colocalization Studies i used Hochest 33342, cell mask and Lysotracker deep red, orange and red. please any one suggest me another lysotracker dye and lysosensore dye according this...
21 February 2024 8,279 0 View
Dear all I'm trying to prepare a lipid bilayer system having more than 100+ no of a specific single lipid. I want to change the conformation of cis bond of lipid into trans form and then perform...
04 February 2024 6,409 0 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View