I misread the amount of time that RNA was stable in RNA later at 4 C as several months instead of a month. I have e zebrafish samples that have taken me a 2 months to collect and they have been sitting in the fridge at 4C. Should I still use these samples for qPCR? I am going to check the RNA quality with a gel and transcription completion with a 5'3' assay, but I am limited on qPCR reagents and don't want to waste them if I'm going to get significant degradation.
Hi,
It is better to check for integrity of samples before going ahead with qRT-PCR
Hi! You coluld verify the integrity of RNA samples with a gel or byoanalizer/tapestation instrument.
I'´ve worked with RNA extracted from FFPE tissue samples and although RNA integrity was fully compromised (as expected), i was able to carry out qPCR analysis. However i`ll highly suggest you (as it has already been mentioned) to check RNA integrity for you to have an idea of how badly degraded your samples are.
I think your RNA will be fine for qPCR analysis, but in your context, I'll definitely check its integrity and quality.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Biomolecules
- Nucleic Acids
- RNA