How to decide or select a signal peptide for the overproduction of monoclonal antibody? what are the factors to be taken care of when selecting the signal peptide? answers with example will be of great help.
Thank you.
have you read about human albumin signal leader ?
https://www.ncbi.nlm.nih.gov/pubmed/23124363
As you consider changing the leader sequence I guess you are not using the hybridoma cells for production. The best sequence depends primarily on the expression system you are using. Using Google Scholar to search for "Recombinant antibody production" https://scholar.google.ch/scholar?hl=en&q=recombinant+antibody+production&btnG=&as_sdt=1%2C5&as_sdtp=
shows many relevant papers, you can further narrow down the search according to your requirements.
You can analysis and verify your signal peptide with SignalP4 (http://www.cbs.dtu.dk/services/SignalP/)
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