I'm working on leakage detection in gas pipelines using OLGA 2015 simulator. I have a problem in using leak component. can anybody help me?

I have simulated a simple 50km pipeline with a leakage in the middle of the line.The flow and Pressure quantities are defined. I have used leak component and set the end time of simulation 48...

23 December 2018 433 2 View

Hello,everybody. can anyone tell me how find optimom time for incubation my cell for transwell migration assay ?

hello,everybody. can anyone tell me how find optimum time for incubation my cell for transwell migration assay ?

08 December 2017 978 2 View

I need books with Bochner Integral, caratheodory and set-valued functions, real and functional analysis, probability theory and stochastic process?

05 September 2016 5,266 15 View

Why adding Cyclohexane (as a porogen) to acrylic monomers like GMA & EGDMA preventing polymerization of these monomers?

13 July 2016 2,772 2 View

How could I measure the specification of these primers?

02 May 2016 1,852 3 View

Which of the packages for solving semidefinite programming in matlab, is better and easy to use?

Semidefinite programming.

06 July 2014 2,564 2 View

Can someone suggest any statistical method to examine the dependency between two variables for a small sample?

I am going to examine the effect of educational achievement on rates of injury for 20 cities for people age 65+. So I have the number of people who was injured in each city, and the number of...

20 July 2013 3,971 3 View

Is giftedness genetically inherited?

I read different research about giftedness and genetic but I would like to ask you about this topic. What do you think?

01 January 1970 5,871 12 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View