I'm working on leakage detection in gas pipelines using OLGA 2015 simulator. I have a problem in using leak component. can anybody help me?

I have simulated a simple 50km pipeline with a leakage in the middle of the line.The flow and Pressure quantities are defined. I have used leak component and set the end time of simulation 48...

23 December 2018 433 2 View

Hello,everybody. can anyone tell me how find optimom time for incubation my cell for transwell migration assay ?

hello,everybody. can anyone tell me how find optimum time for incubation my cell for transwell migration assay ?

08 December 2017 978 2 View

I need books with Bochner Integral, caratheodory and set-valued functions, real and functional analysis, probability theory and stochastic process?

05 September 2016 5,266 15 View

Why adding Cyclohexane (as a porogen) to acrylic monomers like GMA & EGDMA preventing polymerization of these monomers?

13 July 2016 2,772 2 View

How could I measure the specification of these primers?

02 May 2016 1,852 3 View

Which of the packages for solving semidefinite programming in matlab, is better and easy to use?

Semidefinite programming.

06 July 2014 2,564 2 View

Can someone suggest any statistical method to examine the dependency between two variables for a small sample?

I am going to examine the effect of educational achievement on rates of injury for 20 cities for people age 65+. So I have the number of people who was injured in each city, and the number of...

20 July 2013 3,971 3 View

Is giftedness genetically inherited?

I read different research about giftedness and genetic but I would like to ask you about this topic. What do you think?

01 January 1970 5,871 12 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

Why stop the reaction in ice for the fluorescent probe FDA (fluorescein diacetate)?

Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...

08 February 2021 3,366 3 View

What is the possible cause of MSCs low growing?

Hello Everyone, We are inexperienced in culture of MSCs trying to grow a sample from BM of a mare. It is the second time that after preparation of new medium (DMEM-F12 with high glucose - 10% Hy...

07 February 2021 9,989 7 View

Why do cells die after adding MTT dye?

I was doing MTT assay, and in the last day before adding the MTT; the cells looked good (or so I think), but after aspiration of the drug and addition of MTT dye I noticed that most cells seemed...

07 February 2021 3,846 4 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

Why are my western blot bands so distorted?

Hi guys I'm out of ideas here please help! I've never had consistent problem like this doing westerns before and I can't figure it out. The condition used: - First 4 lanes have 50ug protein in...

04 February 2021 2,297 3 View