Dear researchers
What is the best range of IC50? Would you please answer me with the references?
Thank you so much
Roya
Roya Mokhtarian your question does not make any sense. IC is a compound dependent thing. One can't say anything about it without knowing about compound. And as it is a compound specific property, I don't think there is any criteria to say anything about the range in the absence of a reference.
Dear Roya Mokhtarian , It's hard to answer this question as it really depends on the substance you are trying to determine the IC50 for. If you are trying to cover a very large concentration range use a log 10 scale e.g.: 1000, 100, 10, 1, 0.1, 0.01, 0.001 etc. If you have some idea of what the IC50 value should be , based on other groups/publications you can try to narrow the concentration range to suit that. For example, if you know the IC50 of a certain drug is 10 uM, you can have 10 uM as your 'middle' point and then include 3-4 concentrations ablove and below 10 uM. It is important to have concentrations higher and lower than the IC50 so that you are getting complete coverage and you should be able to demonstrate inhibition of >90% for any 'good' inhibitor.
Hi Roya Mokhtarian ,
1. I think your question is not clear.
2. But, in my opinion you want to have an idea of the IC50 range of any particular compound you are planning to work.
3. You wish to see that at which range it has been assessed.
4. Or if you want to known any range of IC50 in general then there is no at all.
5. It is calculated on dose and time dependent manner of any compound usually on any cell line through some specific tests.
Thank you,
Good luck