I would like to work on a project on Mycobacterium tuberculosis, but I am worried about the safe conditions for extracting DNA from Mycobacterium tuberculosis culture.
You can kill the MTB by heating 80°C for 2hrs if solid culture or 80°C for 1hr if liquid culture
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What is the rationale for an adsorbent to adhere to both the Langmuir and Freundlich isotherms in the elimination of methylene blue dye, while remaining unaffected by changes in temperature?
03 October 2023 2,477 2 View
I want to determine the interaction energy between the MgO surface and polymer coating using Materials Studio software. What should be (h k l) cleave plane to have a cut-off distance of at least...
05 February 2023 6,342 1 View
Hi everyone I am using package "XTENDOTHRESDPD" to run a Dynamic panel threshold regression in Stata which is provided here: https://econpapers.repec.org/software/bocbocode/s458745.htm However,...
26 October 2022 5,620 3 View
Dear researchers What is the best range of IC50? Would you please answer me with the references? Thank you so much Roya
14 January 2019 3,081 5 View
I have simulated a simple 50km pipeline with a leakage in the middle of the line.The flow and Pressure quantities are defined. I have used leak component and set the end time of simulation 48...
23 December 2018 577 2 View
hello,everybody. can anyone tell me how find optimum time for incubation my cell for transwell migration assay ?
08 December 2017 1,074 2 View
I am using wet method to fabricate cathode for a lithium ion capacitor. The active materials are AC and LFP, plus AB as conductive additive and PVDF as binder (wt. ratio of 0.25:0.75:0.1:0.1...
16 October 2017 2,320 3 View
We used in our center Virtual Worlds like Secondlife for presentation, networking, sharing and global virtual exchange. Now I would like to know how we could use Virtual Reality to help gifted...
15 February 2017 9,291 8 View
Our virtual world for gifted children: http://world.begabungs.com We are always looking to learn more and share our experiences.
15 February 2017 2,581 1 View
I need some good books that have good examples in each chapters of book. Could you please offer me some good books?
05 September 2016 5,357 15 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
I have been looking at papers for protein expression and trying to follow the process. I'm using TB medium to do the process, and the paper says to grow to an OD600 = 1.8 at 37 degrees, then let...
30 June 2024 6,502 5 View
I am a first-time researcher in the field of nursing and i would like to conduct research about knowledge and attitude towards prevention and spread of tuberculosis.
11 June 2024 3,821 2 View
In 2024, childhood tuberculosis will still be a significant global public health issue, especially in underdeveloped nations (LMICS). It is true that the underestimation of the disease's extent...
31 May 2024 5,221 0 View
I have been trying to check the overexpression of the antitoxin of my chosen TA system of mycobacterium sp. I have tried to induce the clone at 20, 25 and 37° C at 0.1, 1 and 10 mM concentration...
22 May 2024 1,334 0 View
Hello, I have propagated a plasmid form a single colony (size around 15 kb) using small scale culture (2 ml of TB with selected Antibiotic) and purified it using Mini-prep kits ( FastGene Plasmid...
14 May 2024 6,123 2 View
In my undergraduate research project, I require an integrative plasmid containing GFP with an antibiotic resistance that can't be kanamycin. In our lab, all of our integrative-GFP-containing...
14 May 2024 9,861 3 View
We are standardizing chromatographic conditions for first-line TB drugs in our newly procured UHPLC system. For Ethambutol, we are not able to get dedicated chromatogram. Can anyone suggest any...
24 April 2024 9,859 0 View
Hello, everyone. This is Dr Prerna, SR Biochemistry, NITRD, New Delhi. We are developing methods for Therapeutic drug monitoring of first line TB drugs in our laboratory. After utilizing suitable...
17 April 2024 9,598 0 View
I am trying to express a FAD containing enzyme of mycobacteria in E.coli. I am able to purify the protein which is slightly yellow in color but it seems that my FAD is all unbound to the protein....
16 April 2024 6,912 1 View