I'm working on leakage detection in gas pipelines using OLGA 2015 simulator. I have a problem in using leak component. can anybody help me?

I have simulated a simple 50km pipeline with a leakage in the middle of the line.The flow and Pressure quantities are defined. I have used leak component and set the end time of simulation 48...

23 December 2018 433 2 View

Hello,everybody. can anyone tell me how find optimom time for incubation my cell for transwell migration assay ?

hello,everybody. can anyone tell me how find optimum time for incubation my cell for transwell migration assay ?

08 December 2017 978 2 View

I need books with Bochner Integral, caratheodory and set-valued functions, real and functional analysis, probability theory and stochastic process?

05 September 2016 5,266 15 View

Why adding Cyclohexane (as a porogen) to acrylic monomers like GMA & EGDMA preventing polymerization of these monomers?

13 July 2016 2,772 2 View

How could I measure the specification of these primers?

02 May 2016 1,852 3 View

Which of the packages for solving semidefinite programming in matlab, is better and easy to use?

Semidefinite programming.

06 July 2014 2,564 2 View

Can someone suggest any statistical method to examine the dependency between two variables for a small sample?

I am going to examine the effect of educational achievement on rates of injury for 20 cities for people age 65+. So I have the number of people who was injured in each city, and the number of...

20 July 2013 3,971 3 View

Is giftedness genetically inherited?

I read different research about giftedness and genetic but I would like to ask you about this topic. What do you think?

01 January 1970 5,871 12 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Cells not growing in large scale protein expression?

I am using KRX cell. I have grown my overnight cultures at 37 degrees. When I inoculate 1L TB media, my cells do not grow. I have also tried using 25 degree incubation of overnight cultures and...

10 February 2021 3,871 3 View

How to calculate the CFU/ml for Mycobacterium tuberculosis growing in 7H9 broth?

I am running an experiment in which I need to calculate the CFU/ml of a Mycobacterium tuberculosis growing in 7H9 broth. Is there a reliable method to calculate cfu/ml for MTB since a culture of...

22 January 2021 10,027 2 View

How to revive bacteria from glycerol stock?

19 January 2021 7,025 4 View

How to extract and purify disaccharide peptides and lactoyl peptides from Mycobacteria cell wall?

Recently, I try to obtain disaccharide peptides and lactoyl peptides from Mycobacterium Smegmatis, but I cannot succeed. My method is: Disaccharide peptides: The mycobacterial strains were...

12 January 2021 8,352 3 View

Does anyone grow Mycobacteria in TECAN Spark machine to get growth curves? What setting is necessary? Any tips (use Tween20 or tyloxapol?)?

I would like to grow Mycobact. Smegmatis in 24well plate in TECAN Spark machine. So far, I was using 100ml erlenmeyer flasks with 20 ml bacterial culture w/ shaking 180 rpm in 37C . Tecan would...

10 January 2021 6,624 2 View

Does anyone know one antibody that works against Mycobacterium spp?

Anti-Mycobacterium antibody: does anyone know one antibody that works against Mycobacterium spp. (mainly for immunofluorescence)? Many thanks in advance! Cristiane

04 January 2021 9,724 3 View

How long I can store plasma in -80 oC before doing Growth differentiation factor-15 assay ? , Is it different when I stored plasma at -20 or -70 ?

I want to do GDF-15 assay on multidrug resistant tuberculosis patient

29 December 2020 6,764 1 View

What are the invivo screening methods for anti TB drug?

what are the invivo screening methods for anti TB drug?

25 December 2020 722 1 View

Iam looking for articles on mycobacterium tuberculosis?

Hellow my fellows Iam working on Real time PCR technique for detection the gene who is responsiable on Tuberculosis as well as the AFB stain so i hope that any one have worked on the same project...

02 December 2020 2,123 3 View