Genes transient transfected into cells will only be stable for several days. However, xenograft tumors formation need more time about 3 weeks. Whether or not we chould use transient transfected cells for xenograft tumors
Transient transfection of a cell line usually doesn't last for more than a few days, which is not enough for a xenograft study. Our xenografts (http://altogenlabs.com/xenograft-models/) are done with established cell lines, but if you have a given genetic modification you need in a cell line, we can also make a stable cell line (https://altogen.com/services/generation-stably-expressing-cell-lines-28-days/) and use that in a xenograft. The result will be that you have a given genetic characteristic that will not disappear as the tumor grows.
Depending on your gene of interest, a transient knockdown or expression might still reveal interesting mechanistic data (tumor growth, invasion and metastasis). Short term change in gene expression may be amplified in a long term read out like a xenograft tumor model - but maybe not...
Transient transfection of a cell line usually doesn't last for more than a few days, which is not enough for a xenograft study. Our xenografts (http://altogenlabs.com/xenograft-models/) are done with established cell lines, but if you have a given genetic modification you need in a cell line, we can also make a stable cell line (https://altogen.com/services/generation-stably-expressing-cell-lines-28-days/) and use that in a xenograft. The result will be that you have a given genetic characteristic that will not disappear as the tumor grows.
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