I have infected insect cells SF9 with recomb. baculovirus expressing Core.His and another with GFP.
I have extracted the soluble proteins after 3 days and did western blot using anti-his, anti-GFP and anti-tubulin.
the GFP and tubulin gave clear bands at right size but nothing for the Core-His.
I am wondering if I should have extracted insoluble proteins from the pellet? and if so what is the recommended protocol?