How to isolate the pure Ruthenium DMSO complexes from the reaction mixture?

The reaction between N-S bidentate/ONS tridendate ligand (TSC) and cis[RuCl2(DMSO)4] in ethanol by reflux is complete as seen from TLC chromatography. But removing the excess solvent through...

27 December 2020 6,630 1 View

What may be the reasons for broad spectrum in proton NMR (taken in DMSO-d6) for Pd(II) complexes?

The synthesized Pd(II) complexes involved -NH group attached to C=S moiety. The 1H proton NMR taken in DMSO-d6 solvent consisted of a very broad peak at 4.8 ppm with an integration of 6. They are...

09 December 2020 4,340 3 View

What may be the reasons for broad spectrum at 4.8 ppm in proton NMR (taken in DMSO-d6) for Pd(II) complexes?

The synthesized Pd(II) complexes involved -NH group attached to C=S moiety. The 1H proton NMR taken in DMSO-d6 solvent consisted of a very broad peak at 4.8 ppm with an integration of 6. They are...

09 December 2020 9,960 3 View

Why spiro-MeOTAD is not widely used as a hole transport layer in organic solar cells unlike perovskites?

I came across a lot of work regarding the use of spiro as the hole transport material in perovskite solar cell. However, it is not widely used as the HTM in organic solar cells. Can some one tell...

06 November 2019 4,349 4 View

What is the procedure of isolating tissue from canine cancer for performing Proteomics and NGS?

we intend to perform the proteomics analysis and NGS of the isolated tissue from canine cancer model and need the protocol for proteomics and NGS of canine cancer model.

06 October 2019 3,516 3 View

What is the best reaction for forming a conjugate using the compound rose bengal and an amine?

Rose benga is in the disodium salt form and in its structure there is a benzene ring with chlorine atoms,I was wondering if the link can be formed there.

11 March 2017 2,914 1 View

Is it possible to extract RNA from commercial rabies vaccine?

I have tried extraction of RNA from various commercial rabies vaccine which currently used for pre exposure and post exposure for dogs. After that I have done RT- PCR, while running gel i got band...

01 August 2016 1,675 5 View

How can I get a huge dataset for cloud load balancing algorithm to be implemented in CloudSim?

I am working on a load balancing algorithm for cloud in CloudSim with 10 tasks and 4 Vms. But recently, I was asked to work on huge datasets like 100 tasks etc. But in coding, it would be hard to...

28 February 2016 1,065 4 View

In XRD peak, when ever doping materials the peaks will be shifted left to right (or) right to left why?

Ex, Sr doped La2CuO4 in XRD peaks will be shifted right to left.

10 February 2016 5,348 2 View

How long can I culture HUVECs in a 6 well plate?

22 August 2014 2,380 7 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View