Nithya Sukumar

2 Questions 1 Answers 0 Followers

Questions related from Nithya Sukumar

Should I use phospho-AKT Ser473 or Thr308 antibody in Western blot?

I want to look at the phosphorylation of AKT in HUVECs treated with hyperglycaemia. Can anyone suggest whether I should use the pAKT Ser473 or Thr308 antibody? Does it matter which one of the...

24 October 2014 6,561 1 View

How long can I culture HUVECs in a 6 well plate?

I need to treat HUVEC cells with different glucose concentrations for up to 14 days. How do I do this in a 6 well plate because the cells will reach confluence within a few days? Can I use trpysin...

23 August 2014 2,667 7 View