What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Why differs CT values of one sample between its triplos (Taqman Assays and qPCR)?

At first, we performed transfections. To analyze that transfection, we performed RNA isolation, cDNA synthesis and qPCR. Using the qPCR, we analyze multiple samples, whereof each sample we...

19 March 2020 2,301 4 View

What is the optimal method to the cryopreservation of SH-SY5Y cells?

I have tried multiple times to cryopreservation of SH-SY5Y cells, but afther thawing, no cells had survived. I bring the cells to cold (4 degrees Celsius) DMEM/F-12 medium with 5% DMSO. After...

14 November 2019 3,474 3 View

What data analysis tools can be used in order to validate data quality?

I am looking for data analysis tools that can be added into a new database (legacy system migration, without database from legacy system) which takes structured data (pre-determined format, seen...

25 March 2018 269 3 View

What should I do when comparing response frequencies in multiple response items?

I have a multiple response item consisting of 10 response options. Participants were instructed to choose 3 of them. I want to know which one of those response options was picked the most. Of...

06 December 2017 6,047 6 View

How to reduce off-target effects siRNA?

28 November 2016 2,726 8 View

How to incorporate thymidine analogs by PCR?

I am aiming to create 5 - 10 kb amplicons by PCR with all thymidines replaced by different analogs (i.e. BrdU, EdU, CldU, IdU). For this, I tried to simply replace dTTP by dBrdU (or other) in the...

29 June 2016 9,892 1 View

How to perform this Granger causality test?

I want to do research on the effect of international migration on wages and productivity in the construction industry in the Netherlands. My supervisor suggested a Granger causality test. I...

24 April 2016 8,235 3 View

What are good alternatives for rhizon samplers to sample pore water from heavy clay sediments?

11 April 2016 3,949 3 View

Are there Pollen datasets for your country to test its inverse effect on Covid-19/flu-like incidence?

We recently found - using Dutch multi-year datasets - that pollen are likely the seasonal factor in inhibiting flu-like epidemics (see below). We tested it for 2016-2019 and will expand that...

01 January 1970 7,338 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Can anyone advise me about a cortisol antibody made in rabbit to set up an ELISA?

I am trying to set up a cortisol EIA using mouse anti-rabbit IGg as coating and a rabbit cortisol antibody as secondary ab. I am searching for a sensitive and specific cortisol Ab made in rabbit

20 February 2021 3,366 5 View