I ve started with culturing HCN-2 cells. I start this by coating 10 cm dish with Poly-L-ornithine for 24 hours. After that, I removed the excess liquid and washed one time with PBS. After that, I added the cells to the plate. Does anyone now how long it take for the HCN-2 cells for attaching to the coating or did I anything wrong using the coating? After 2 days past adding the cells to the plate, 1/3 of the cells are on the bottom of the plate, but I am not sure that cells are attached.