Should i perform transcardial perfusion before lung isolation for lung inflammation analysis ?

11 January 2021 1 6K Report

i'm working on Emphysema animal model, i'm looking for lung inflamamtion and alveolar wall destruction , is it necessary to do lung perfusion with PBS before lung isolation ? to ovoid having blood in my histological slides

i'm wondering also if the perfusion can affect the lung inflammation and alveolar wall architecture ?

thank you

Yuansheng Liu

It is suggested that you‘d better divide each group into two parts, one part of the animals to do alveolar lavage and the other part to take the organs directly

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