Slama Nada

4 Questions 7 Answers 0 Followers

Questions related from Slama Nada

Why are bronchoalveolar lavage cells are lysed?

hi, after performing bronchoalveolar lavage in mice with PBS, I use trypan and MGG coloration to see and count the inflammatory cells in my samples, it seems like the cells are lysed, i don't know...

25 April 2021 4,033 0 View

Should i perform transcardial perfusion before lung isolation for lung inflammation analysis ?

i'm working on Emphysema animal model, i'm looking for lung inflamamtion and alveolar wall destruction , is it necessary to do lung perfusion with PBS before lung isolation ? to ovoid having...

11 January 2021 5,830 1 View

Can anyone help me, i am trying to calculate mean linear intercept in my histological slides ?

in the literature, in order to calculate this parameter , they use slide images with 20 microscopic magnification , but in our lab , we don't have this kind of microscope , can I use 10...

12 December 2020 9,788 3 View

Can anyone help me with the protocol of intranasal administration to mice ?

i wanna administer cigarette smoke extract to induce emphysema in mice, i am asking for the maximum volume of intranasal administration ? can i use ether or chlorofom to anesthetize mice?

11 December 2020 6,470 4 View