What imaging modalities are useful in assessing myocardial perfusion and viability?

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Detection and Identification of Amanitins in the Wood-Rotting Fungi Galerina fasciculata andGalerina helvoliceps

Dear all, Can anyone help me to contact the corresponding author of the paper " Muraoka, S., Fukamachi, N., Mizumoto, K., & Shinozawa, T. (1999). Detection and Identification of Amanitins in...

01 January 1970 6,517 3 View

Edible mushrooms and their cultivation

Hi All, Does anyone have the PDF of following book? Chang, S. T.; Miles, P. G. (1989). Edible mushrooms and their cultivation. Edible Mushrooms and Their Cultivation, 345. Thanks

01 January 1970 7,851 1 View

Is it possible to perform cardiac puncture followed by PBS perfusion with a pump?

Hello all, I will be perfusing mice with PBS for brain harvest and a colleague would like to collect the blood via cardiac puncture (these are 18m old mice so would be nice to maximize use). Has...

15 February 2021 5,312 2 View

Is it better to push or pull liquid through microfluidics with a syringe pump?

I have a PDMS microfluidic device that I want to perfuse cells through (there are also adherent cells in the channels). Is there any difference if I use a syringe pump to push the cells through...

30 January 2021 4,092 3 View

What is the best antibody to identify human cells in mouse brain?

Dear colleagues, We transplant human cells in the mouse brain. After perfusion with 4% PFA (+ 4-6 hours post-fix) we want to identify histologically the human cells in a brain section. We have...

19 January 2021 352 3 View

Thin cut brain tissue flaking off of slides?

Hello! I have just encountered a new issue with some tissue flaking off of my slides and wondered if anyone else has experienced this or has any suggestions? The sections were cut at 18 microns...

09 December 2020 4,954 2 View

Mouse brain extraction without fixation perfusion?

I am currently working on a mouse model that I will be collecting long bones and muscles from. We have decided to extract the brain for IHC and we do not want to perform transcardial perfusion as...

12 November 2020 5,821 4 View

Does anyone know of a reliable source for primary antibodies against oxytocin and vasopressin for IHC studies in rats?

The rats are perfused with PBS and the brain sections are 18 microns thick and fixed on slides.

09 November 2020 833 3 View

Does PFA transcardial perfusion artefactually stretch the rodent heart?

Hi everyone I'm comparing aorta/ventricle wall thickness of mouse. Perfusion was done to minimise auto fluorescence during confocal IF. Question is whether transcardial perfusion artefactually...

28 October 2020 8,059 2 View

Should I perform transcardial perfusion by PBS before lung isolation for RNA expression analysis?

Hey, I am planning to dissect rats and isolate Lung tissue samples to be stored in RNA stabilizer for later analysis. - Should I perform trandcardial perfusion by PBS ? - Is it necessary to...

21 September 2020 1,822 5 View

Is perfusion neccessary for brain IHC analysis?

Hi, we are generating primary neurons - I was curious if the remainder of the brain could be fixed in PFA and used for IHC analysis without prior perfusion fixation?

21 September 2020 4,358 3 View

How do you check the locations of brain-implanted cannulas?

Hello. I implanted single cannulas bilaterally into the brains of mice to infuse drugs. After behavior tests, what would be the simplese way to validate if the cannulas are in the target areas?...

18 September 2020 8,457 3 View