isolated from water and sediments samples.
I have a protein that is 117 amino acids long, with a 21 amino acid transmembrane domain at the C-terminus. I've cloned this protein into the pET28 expression vector in BL21, but am not seeing any...
20 May 2024 8,161 2 View
My research was published in the Italian magazine Scopus Q2 in 2021. The attachments are my research and the research of Indian researchers in 2023.
01 April 2024 5,153 3 View
I am doing research, and one of my research variables is audit risk assessment. I want to do applied studies instead of questionnaires.
18 March 2024 9,997 3 View
What is expected of future generations towards society and the nation ? What are the trends that generations aim to achieve ? for example a peaceful generation .. believes in values.. a...
02 January 2024 7,324 2 View
there is a problem in my code and i dont know what is it ? if there is available code can you help me with it
17 December 2023 7,151 0 View
Explanation of Time Series Research Design
19 November 2023 5,721 2 View
Among the tools of scientific research, quantitative and statistics..
12 September 2023 2,367 4 View
I am amplifying gene (1488 bp) from RNA genome using specific primers and run in reverse transcription PCR(RT-PCR). The result, I am getting 2 bands my target band (1488bp) and extra band with...
01 September 2023 4,965 7 View
Hi, I has a question regarding infection models- If you are inducing an infection model (bacterial) and then snap freezing the infected tissues in liquid nitrogen afterwards, does the pathogen...
18 July 2023 4,672 1 View
I have milk digesta and I need to quantify free fatty acids using FAME with GCMS. Can I extract the lipid fraction by Folch method then inject directly without further extraction of the free fatty...
18 June 2023 5,286 2 View
Hi researchers! I'm working on soil texture analysis, and the end result for sand is doubtful because there is black sediment appearing after drying, as shown in the figure. Is it considered sand?...
30 July 2024 557 2 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...
25 July 2024 2,532 3 View
Hello, I am experiencing an issue with mixing micro powder with resin in a 3D printer. The problem is that sedimentation occurs after a short period (about half an hour to an hour). Could you...
23 July 2024 1,672 0 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...
15 July 2024 5,898 0 View
Low magenic concenrartion indicating enhanced chemical weathering associated with warm and wet climate in detrital sediments.
14 July 2024 1,381 4 View
I have done the same procedure with BL21 and pLysE but i got colonies but multiple times on rosetta i did but not getting. can anyone explain what would have gone wrong?
12 July 2024 9,904 1 View
I am working on probiotic bacteria for fish, and I am trying to isolate probiotic bacteria like lactic acid bacteria or other probiotics from the gastrointestinal tract of fish using different...
12 July 2024 3,756 4 View
How can we measure their colony diameter?
12 July 2024 7,027 0 View