isolated from water and sediments samples.
What do you know about biotechnology art?
02 March 2021 1,653 3 View
in the literature, in order to calculate this parameter , they use slide images with 20 microscopic magnification , but in our lab , we don't have this kind of microscope , can I use 10...
11 December 2020 9,559 3 View
i wanna administer cigarette smoke extract to induce emphysema in mice, i am asking for the maximum volume of intranasal administration ? can i use ether or chlorofom to anesthetize mice?
10 December 2020 6,319 4 View
In a scholar profile, in google scholar, when clicking on a link of an article for example, why some articles have a description and link to the page of the journal that publishes that article...
16 November 2020 5,216 4 View
Hello everyone, Hope you are very well. I just wonder if there is a word or LaTex Elsevier template for these two...
06 November 2020 4,504 3 View
Hybride intelligent system is good techniques used for solving diffrent optomization problems. Like classification, recognition ....etc Deep learning can be used with and hybrid with other machine...
08 October 2020 4,943 3 View
As hydrogel was prepared in acidic medium, released acid from hydrogel kills cells in in-vitro test. I am wondering to compromise such issue.
12 August 2020 9,319 3 View
Dear all, I am currently performing some western blots in a big wells gel (40ul capacity) and i am filling 40ul in each well. My problem is that two of the last wells supposed to be empty...
10 August 2020 4,613 4 View
mention the name of parasitic worm that causes river blindness?
04 July 2020 6,066 8 View
I have daily closing values of multiple stock indices and i have lists of public holidays for the whole period. I want to inspect the existence of a holiday effect. My regression equation is as...
23 May 2020 5,456 12 View
Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...
01 March 2021 2,027 2 View
Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...
28 February 2021 7,292 3 View
I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...
25 February 2021 3,221 7 View
Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...
24 February 2021 9,839 3 View
Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...
24 February 2021 5,759 3 View
Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...
16 February 2021 1,664 3 View
I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...
16 February 2021 169 4 View
Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...
14 February 2021 2,039 4 View
I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...
09 February 2021 4,158 1 View
03 February 2021 1,111 5 View
