During the subculturing of an MCF-7 cell line, the cells were trypsinized and transferred to a new flask. Initially, the cells grew normally, with no unusual objects observed. However, after one week, when the cells became confluent, black structures appeared. Following another round of trypsinization, these structures became more distinct. Throughout this period, the medium remained stable, showing no changes in pH or turbidity.
Could these structures indicate contamination?
Dear Mrs.Naraghi
Do your cells medium showing turbidity on the flask?
Do the black structure moving?
From my experience, contaminated cells usually have round or oval object that moving and the cell mediums is showing turbidity. I actually doesnt really know which black object do u mean, I guess the object that looks like branch, right?.
My suggestions are try to brighter the microscope light, check for the flask or the lens if there is scratch. You also can try to incubate your cell using anti mycotic anti bacteria for 2 hours to eliminate the bacteria/fungi contamination :)
Rizky Clarinta Putri Thanks for your response. No turbidity was observed and the branched black objects did not move.
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