Houra Naraghi

11 Questions 16 Answers 0 Followers

Questions related from Houra Naraghi

How to prepare a transparent solution of the peptide with the mixture of hydrophobic and hydrophilic amino acids ?

I am using a peptide and have faced challenges in preparing a transparent peptide solution. My goal is to prepare a peptide solution in MES buffer and then use the solution to immobilize the...

21 June 2024 8,998 5 View

How to choose a release medium for paclitaxel?

Exploring the release rate of paclitaxel from a mesoporous silica nanoparticle, prior studies have employed various release mediums, such as PBS buffer, or PBS supplemented with Tween or SDS at...

08 March 2024 4,597 2 View

Dissolution test- How to choose a release medium for paclitaxel?

Exploring the release rate of paclitaxel from a mesoporous silica nanoparticle, prior studies have employed various release mediums, such as PBS buffer, or PBS supplemented with Tween or SDS at...

12 February 2024 5,531 2 View

How can I ascertain the absorbance of the analyte considering the presence of matrix effects?

I have a peptide dissolved in DMSO and MES buffer, and I aim to determine its absorbance using UV-Vis spectroscopy. However, I'm encountering issues with obtaining meaningful results at 220 nm,...

30 November 2023 341 3 View

How can I dissolve the peptide ?

I have an acidic peptide with 12aa (containing 5 hydrophobic amino acids), disulfide bridge, Methionine, and amidation at the C-terminal. I have tried dissolving using acetonitrile, NH4HCO3 0.1 M,...

10 July 2023 3,062 6 View

Peptide amount estimation for designing a targeted drug delivery system ?

I want to design a drug delivery system using peptides as targeting ligands which are supposed to interact with cell surface receptors. Has anyone experienced a similar experiment to help with the...

16 April 2023 6,914 0 View

Designing a targeted drug delivery system-Peptide amount estimation ?

I want to design a drug delivery system using peptides as targeting ligands which are supposed to interact with cell surface receptors. Has anyone experienced a similar experiment to help with the...

08 February 2023 2,419 0 View

Synthetic peptide analysis using HPLC and MALDI ?

I synthesized a short hydrophobic peptide and confirmed its synthesis using MALDI_TOF analysis for the crude sample. Before purifying using a semi-prep column the sample was injected into the...

19 January 2023 1,144 6 View

How to lable mesoporous silica nanoparticles with FAM?

Dear all I want to lable mesoporous silica nanoparticles with the 5(6)-Carboxyfluorescein (FAM) dye. Do you have any suggestions about the following: - What is the functional group of FAM? Is...

31 August 2021 4,977 4 View

peptide properties calculation ?

How can I find peptide properties like its surface area, volume, anchoring amino acids for binding,? Is there any data bank or online calculator for this purpose?

25 March 2021 7,766 6 View

Should I consider these black objects as cell culture contamination?

During the subculturing of an MCF-7 cell line, the cells were trypsinized and transferred to a new flask. Initially, the cells grew normally, with no unusual objects observed. However, after one...

01 January 1970 9,461 4 View