I have an acidic peptide with 12aa (containing 5 hydrophobic amino acids), disulfide bridge, Methionine, and amidation at the C-terminal. I have tried dissolving using acetonitrile, NH4HCO3 0.1 M, and 2.5% ammonia solution separately. However, I can still see some tiny particles in the solution, which is a little turbid.
How can I dissolve the peptide easily?
How can I be sure about the non-dissolved particles? Are they peptides or can they be impurities?
Try DMSO, DMF or NMP to make a stock, then add water in tiny portions.
You should also use Methanol instead of Acetonitrile (which is known to precipitate proteins/peptides).
Thanks for your time and your suggestions Wolfgang Schechinger Bruce Neagle
Comparing peptide solubility using different solvents the result was as follows:
Ammounia and DMF>DMSO>NH4HCO3
Ammonia 0.1 %
NH4HCO3 0.1 M
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