In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein?

Hi In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein? I got this error in the add-ion stage: Fatal error "./ amber99sb-ildn-HHH.ff / force...

01 March 2021 8,747 4 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Why CNN algorithm performs well in Network Intrusion Detection based on some recent resources?

Most of the papers have compared different hyperparameters such as layers, initializers, activation function, and optimizer algorithm in the CNN algorithm for detecting network intrusion. This is...

24 February 2021 4,578 4 View

Historical wind data/map?

Hello All, I am looking for the historical wind data/map (mainly the year 2020-2021). I would like to look at the wind value and direction for the specifics locations within the US for the...

18 February 2021 9,543 4 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

The reason of PMMA channels cracking?

07 February 2021 3,663 2 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

How to solve failure of Fluent calculation (Error: floating point exception / Error Object: #f)?

Hi everybody I'm stuck with this error and I dont know what to do to solve this problem Initialization is ok but when I start my calculation this error appears (Error: floating point exception /...

25 January 2021 8,091 3 View

How to best prepare cells for FACS from live tissue?

Hi everyone, I'm currently trying to perform single cell RNA seq on a small (

24 January 2021 9,804 2 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

Does anybody know the ways of homogeneous ripening of Mung bean crop?

Some crops (Mung bean) are matured at different times i.e., harvested at several times that's why the labour cost of the farmer is high. So, could you please, is there any ways or chemicals are...

12 February 2021 294 4 View

How much could knowing the maturity level of an organization contribute to a given process?

The doubt is raised because there are several methodologies to estimate the degree of maturity of certain processes, such as computerization, communication, human resources, among others, and...

03 February 2021 4,753 7 View

Is there any correlation between mature miRNAs and their precursors?

I have a series of RNA-seq datasets that their average spot length is about 90bp. (don't include small RNA reads like mature miRNAs). I'd like to do "Gene" and "precursor miRNAs" expression...

07 December 2020 7,744 3 View

Hello every one! How can i design the primer for mature microRNA for RT-PCR quantification?

I am going to validate some microRNA genes in two developmental stages. But i found the method for miRNA primer designing is totally change compering to mRNA and LncRNA primers designing. Please...

03 December 2020 4,555 6 View

Help in Sample Size Calculation for my Thesis Comparing the Clinical Outcome of Pulpotomy in Mature Permanent Teeth with MTA & EBRRM?

I am looking for assistance in finding statistics for sample size calculation on topic Comparing the Clinical Outcome of Complete Pulpotomy in Mature Permanent Teeth using MTA & Endosequence...

20 October 2020 8,197 6 View

MiRNA seq differential analysis?

Hello, I am writing to ask your help in regard to performing differential analysis to analyze miR RNA-seq data. I have miRNA seq data and I have analyzed in the following way- Initial QC - removed...

19 August 2020 5,105 7 View

Please i'm a new user of SAS, I need the steps to analyze a soybean data, comparing same dependent variables of 15 locations?

The design is RCBD, 16 entries, 3 reps, 4 blocks, Name. The dependent variables are date of flowering, Flower Color, Date of maturity, Daymat, Grainkha Pop_000, Dayfl, Shat2wk, Shat3wk,...

14 June 2020 571 4 View

Finding relationship between independent variables fixed per condition from a maturity model and dependent variables Likert scales from a survey?

Hello I have a question regarding a dataset I must analyze. I did two research steps: 1. Based on a managerial maturity model, I ranked 8 brands according to their level of usage of social media...

08 May 2020 7,905 6 View

Covariate in multiple regression?

I want to examine the relationship of various physical qualities (jump height, change of direction, linear sprint, etc.) on agility performance. Because I am interested in the combined effect of...

07 May 2020 3,092 10 View