Which Tm should I use in my PCR ?

I have ordered primers from Thermo fisher scientific and in the analysis sheet it has been mentioned two tm The first one is tm 1M na+ 70 and the second is Tm 50mM na+ 48 and each has different...

29 August 2020 6,890 12 View

How i can Improve the accuracy of an artificial intelligence model for test data in MATLAB ?

Hello everyone, I have a question about artificial intelligence in MATLAB. Can you help me, to improve the accuracy of an artificial intelligence model for test data in MATLAB software? (while...

22 July 2020 2,692 12 View

Does anyone know how can I get Mito Thermo Yellow (MTY) dye?

This is a thermosensitive dye that was recently used by chretien et al to measure changes in mitochondrial temperatures.

01 March 2020 4,863 4 View

How to continue after tissue processor failure (samples left to dry for hours)?

For some reason our tissue processor had stopped working during last night, leaving my samples (different murine tissues fixed in 4% PFA o/n) in the air before the last ABS-ethanol. I put the...

20 January 2020 523 2 View

If a staph bacteria shows resistance to vancomycin disk on Mueller Hinton plates, does it mean its Methecillin resistance?

Also can staph aureus bacteria appear pink on Manitol salt agar plates? Or it definitely appears yellow? Has anyone isolated staph aureus with alpha hemolytic characteristics? Thanks.

06 February 2019 409 4 View

How to use yalom existential psychotherapy or group therapy in school setting?

Psychotherapy can help most patients confront the issue of responsibility for their own life, problems, and improvement (Yalom, 1974). Group therapy can be somewhat beneficial for clients who are...

12 December 2018 6,850 3 View

What are the safety requirements when using nano-carbon tubes for reinforcing Polymeric matrix?

Hi for All I need your recommendations from safety requirements when using nano-carbon tubes for reinforcing Polymeric matrix. Thanks in advance

06 July 2018 326 2 View

Do you know the latest list of the journals that discontinued from Scopus?

Hi dear colleagues Do you know the latest list of the journals that discontinued from Scopus? You can see it in this file which downloaded from Scopus website (last updated 26/3/2018). Regards

14 April 2018 4,977 17 View

From the copyright viewpoint, is it legally permitted for the researcher to add the full text of the published paper on the research gate platform?

Hi dear colleagues. From the copyright viewpoint for journals, is it legally permitted for the researcher to add the full text of the published paper on the research gate platform? This question...

05 April 2018 9,253 74 View

Is there any useful book or standard to interpret the concrete microstructure tests (for example SEM, XRD,....etc.) results?

Hi dear colleagues, Is there any useful book or standard to interpret the concrete microstructure tests (for example SEM, XRD,....etc.) results? Regards

28 March 2018 9,546 13 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View

Can you advise reference publications/standards on characterizing animal genetics research and development activities, and the outputs it generates?

I am doing semantic analysis of science and grey literature publications in the field of animal genetics and breeding. I have a standard dictionary but I am not sure if it captures the state of...

31 December 2020 4,924 3 View

How can i perform gene overexpression study in vivo? in sheep or mice model?

I am planning to transfect/infect a target gene directly into sheep or mice body to study its effect. need guidance please

03 December 2020 434 4 View

Who can get this manuscript " Development of SYBR Green real-time PCR for diagnosis of fasciolosis in sheep"?

30 October 2020 9,456 2 View

Do the tannic acid-treated sheep RBCs show agglutination with FAdVs (fowl adenoviruses)?

As we know that Adenoviridae indirectly agglutinates the RBCs of chicken, mice, rhesus monkey, and human blood type "O".However, do the tannic acid-treated sheep RBC show agglutination with FAdVs...

17 September 2020 6,630 3 View

Literature on traditional/ancient slaughtering patterns of goats/sheep?

Any recommendations? I'm mostly (but not exlusively) interested in eastern Mediterranean and West Asia...

09 September 2020 7,344 3 View

Can AI solve the viral Chinese Math Problem for 5th grade students concerning the minimal age of the captain?

28 February 2020 5,998 5 View

I am doing MIC susceptibility test on S. mutans. Can i culture them on Brain heart infusion then pour them on Mueller hinton agar plate?

I am doing MIC susceptibility test on S. mutans. Can i culture them on Brain heart infusion then pour them on Mueller hinton agar plate then do the susceptibility test after? Also, where can i...

14 February 2020 6,504 2 View

I am doing MIC susceptibility test on S. mutans. Can i culture them on Brain heart infusion then pour them on Mueller hinton agar plate?

I am doing MIC susceptibility test on S. mutans. Can i culture them on Brain heart infusion then pour them on Mueller hinton agar plate then do the susceptibility test after? Also, where can i...

01 February 2020 5,919 2 View