I am first time doing bacteria experiment without any prior practice. I am trying to make bifidobacterium biofilm. According to references i am incubating them in different concentration of bile acid+BL broth (2hr~24hr) and then spreading them on BL agar plate containing sheep blood.
While getting normal colonies in control plate some plate is showing white patch like the image. I am not sure if it's biofilm or just contamination. The growth condition was 5% co2, 99.9% nitrogen and 0.1% oxygen.
I scrapped some of the patch and tried to check 40x optical microscope but can not conclude anything. As i have no basic training training about bacterial experiments i am having hard time.
Thanks a lot.
@Phil Geis
Usually the bifidobacterium colony appears much smaller is size and opaque in color. You can see some colonies beside the big white thing. If it's a colony why it changed shape and color? Because of EPS? Any explanation will be very helpful. Thanks.
Colony morphology differs between microbes. There appear to be two different isolates. EPS would not per se be responsible for the larger colony.
See at link below a colony displaying a large amount of EPS
https://www.microbiologyinpictures.com/bacteria-photos/klebsiella-pneumoniae-photos/klebsiella-escherichia-endo.jpg
Compare the morphology of bifedobacterium with your plates colonies under microscope
You may look into the article here. Hope it helps
https://doi.org/10.1007/s13762-024-05635-3
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