I am extracting rna, from sheep goat blood with rna later, I use RNeasy Plus Universal Mini Kit (50) however while I get a good ratio of 280/260, the concentration is very low, does anyone know what blood concentrations and QIAzol Lysis Reagent I need to get a good concentration; Thank you very much
To improve your RNA yield, ensure you're using an appropriate amount of blood and QIAzol Lysis Reagent. Generally, for blood samples, a starting ratio of 1 mL of blood to 3 mL of QIAzol is recommended. Also, consider optimizing your RNA extraction protocol by adjusting parameters such as centrifugation speed and duration, as well as the volume of elution buffer used. Additionally, factors like the age of the blood sample and the health of the animals can affect RNA yield.
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