Dear all,
I am maintaining sheep iPSCs, however after few passages sometimes they start making mysterious projection which are not characteristic of these iPSC. I am following best practices to minimize any kind of stress to these cells (light, temperature difference, changing media daily). These happens to my cells only, as other team members grow cells in good state. These are earlier in passage (p18), doesn't happen every time. However, quite frequent, I discard these cells and start over. Need your suggestions and explanation for this weird phenomena. I have attached pictures at 4x, 10x and 20x.
Thanks in advance for your time