Gene of my interest has exactly similarity to pseudogene.Can i still design the real time primer in same region,my question is do pseudogene will have impact on the detection original transcript levels.
Or we can assume pseudogene wont intefere .
Hi Satyajeet,
In some cases pseudogenes won't interfere with your qPCR analysis, and that is when the pseudogenes are not transcribed. However, chances are this pseudogene is transcribed, so it can affect your real time based assay, but only can not be translated into a functional protein. That being said, when you design qPCR primer sets, try to target the regions that make the pseudogenes "pseudo", i.e., aim at the difference between the g.o.i and its pseudogene.
ScienCell Research Laboratories carries primer sets for over 2,000 genes for qPCR gene expression profiling. All our primer sets are designed by our PhD scientist team and are wet-lab verified. We also offer custom primer design and verification services. For more information, please go to: http://www.sciencellonline.com/products-services/gene-expression-profiling.html
Best regards,
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