I am performing a luciferase transfection in U87 cell lines to express the luciferin gene. I cultured them in a 24-well plate, and pulled A+B to 1 10-cm dish, then pulled C+D to 1 10-cm dish. My question, I am trying to figure out the dosage of my antibiotics, G418 (Geneticin). If my stock concentration is 50mg/mL and I want to dose with 800ug/mL in 10mL of media for a 10-cm dish. What is the math that I need to convert this over and get my volume (uL) of how much I should treat my cells to select for the luciferase expression amongst my cells.
Normally you would be calculating the amount needed on the final concentration you want to achieve x total volume / stock concentration. However you state in your question that you want 25 mg per dish. If that is actually correct (25 mg per dish) and your stock is 50mg/ml then you need to add 0.5mL (or 500uL) per dish of your stock. But are you sure that is the amount you want per plate, or is it really a concentration per mL of medium (which is more normal)? Also 25mg sounds pretty high to me.
Danisha Davis I agree with Artur Burzynski
that you should ask for some help with how to do these calculations in case you aren't giving us the right information. But I provided the equation you need to use. Final concentration x volume / Stock concentration. Just be sure your units are all the same. You provided the stock concentration (50mg/ml) and the final concentration (0.8mg/ml) but not the volume. Also it is not clear what the 25mg per plate is meant to represent.- Badges
- Science method