Danisha Davis

3 Questions 2 Answers 0 Followers

Questions related from Danisha Davis

Invasion Assay with HCC cell lines, unsure why we seen very few cells in 1% crystal violet staining?

Essentially, we have various HCC cell lines that we plan to do a migration assay using corning transwell 8.0 um pores w/ matrigel. Utilized a chemoattractant of 5% FBS/DMEM serum as the...

01 March 2022 5,807 2 View

How do I fixate cells without violet staining in a wound healing assay?

Working on some cell culture & different lines, does anyone have a specific protocol for fixing cells with formalin & using Triton X-100 to fix cells?

29 December 2021 4,345 1 View

Selective Antibiotic G418 in Cell Culture (Math) What is my volume (uL) of how much I should treat my cells?

I am performing a luciferase transfection in U87 cell lines to express the luciferin gene. I cultured them in a 24-well plate, and pulled A+B to 1 10-cm dish, then pulled C+D to 1 10-cm dish. My...

09 November 2021 8,401 3 View