16 January 2019 4 4K Report

Hi,

After immunoprecipitation and elution, I check the eluate with western blot. If I incubate the blot ONLY with secondary antibody, it gives the same signal as incubatin with primary & secondary?

Is it because the secondary is "sticky" and will bind to membrane-rich patches in the membrane?

How to validate if I immunoprecipitated the protein, if the secondary also gives a signal without the primary?

Thanks a lot!

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