9 Questions 15 Answers 0 Followers
Questions related from Stijn Vdb
Hi, Which lysis buffer would you recommend to make a protein extract from tissue that does not interfere with immunoprecipitation afterwards? I'm currently using RIPA + protease inhibitors,...
21 October 2019 8,938 5 View
Hi all, Is it possible that the migration of my protein is delayed due to the complexity of the sample? When I run a western blot with 1) a protein extract from locust tissue and 2) hemolymph...
04 June 2019 10,020 10 View
Hi, I've performed a protein extraction on different insect tissues using a 2D DIGE lysis buffer (containing CHAPS, ureum, thioureum and DTT). When performing a BCA assay, my samples (which often...
02 April 2019 7,540 6 View
Hi, After immunoprecipitation and elution, I check the eluate with western blot. If I incubate the blot ONLY with secondary antibody, it gives the same signal as incubatin with primary &...
16 January 2019 3,970 4 View
Hello, So, first I perform immunoprecipitation. The eluate is then subjected to immunoblot analysis using an antibody against a related protein (equal size) and gives a band. How sure am I the...
14 November 2018 5,501 7 View
Can someone give me an easy example of how to normalize qPCR data against spike-in control? Thanks
06 March 2018 9,667 1 View
Hello, Is it possible to separate a complex fluid in a vesicular part (containing vesicles / exosomes) and a non - vesicular part, containing other proteins? Many thanks!
29 June 2017 5,612 3 View
Hello, What is the function of XBP buffer in exosome isolation? Does it solely bind to exosomes, or also other protein complexes?
08 May 2017 3,471 2 View
Hello, Does anyone has experience with extracting hemolymph from the pupa stage of small insects (not Manduca) but for example Drosophila or Spodoptera species? Thank you!
19 October 2016 4,602 2 View
