How can I prepare 10X SDS Sample Buffer? Can you give me the recipe?
Here you go.
It's 4X concentration. Use it in 1X with your sample and heat (boil) the mixture well to denaturation.
I agree with @jitendra. Making more concentrated sample buffer is just not possible, nor necessary. The difference in detection sensitivity between a 0.75 (4X sample buffer) and a 0.9 (10X sample buffer) dilution is not significant.
(you might buy the buffer if you wish)
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