Hi all,
I've recently been culturing primary rat schwann cells to prepare for experiments. The schwann cells looked fine during my previous subculture in a T-75 flask. Upon new subculturing and seeding unto a 96-well flask I found the following ( in attached image). The cells are currently on P7. My PI thinks it is a bacterial infection. I don't totally agree. I've asked other PhD students and candidates and they think its signs of debris and not contamination per-say.
The media did not change colors abruptly, and the cells do not move ( after looking at it under the microscope for one minute). I tried to wash with PBS but very little changed.
Has anyone seen this before? Would you classify this as contamination or debris of some sort
On left side is infection from fungus kind of material and on right side would be degradation of cells (I can not think of the reason, either media dried on that side on the tilted plate)
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