Chukwuweike Uchenna Gwam

4 Questions 4 Answers 0 Followers

Questions related from Chukwuweike Uchenna Gwam

Schwann cell culture contamination, debris or apoptosis?

Hi all, I've recently been culturing primary rat schwann cells to prepare for experiments. The schwann cells looked fine during my previous subculture in a T-75 flask. Upon new subculturing...

10 October 2019 3,859 2 View

Does acute oxidative stress activate schwann cell proliferation?

Hi all, I am running a n experiment involving primary rat Schwann cells ( passage 3) and varying dosages of H202 (0mM, 0.1mM, 0.3mM, 0.8mM, 1mM, and 2mM). Schwann cells ( n=2,000) were grown on...

07 July 2019 2,725 5 View

Does skeletal muscle stain positive for collagen?

Hi all, I did trichrome staining for dennervated rat muscle. The results demonstrated positive aniline blue staining in the muscle at about 2 to 5% of the surface area. My question is does...

06 June 2019 630 3 View

Way to model denervation in-vitro?

Hi all, I'm looking to recapitulate denervation in-vitro using mice myotubes (C2C12). Is there a way to best recapitulate denervation ( with the obvious limitations of being in-vitro)...

05 May 2019 1,532 1 View