Way to model denervation in-vitro?

Chukwuweike Uchenna Gwam @Chukwuweike_Gwam2

05 May 2019 1 2K Report

Hi all,

I'm looking to recapitulate denervation in-vitro using mice myotubes (C2C12). Is there a way to best recapitulate denervation ( with the obvious limitations of being in-vitro) in-vitro that anyone is aware of?

Thanks

Ayman Khalifa

Hi Chukwuweike Uchenna Gwam

Did you find any way to denervate invitro

I am looking for denevate mice skin invitro and hope yo caught a good way .

Thanks

Badges
Science topic

Similar topics
Eutheria
Rodentia
Muridae
Murinae
Mice

More Chukwuweike Uchenna Gwam's questions See All

Schwann cell culture contamination, debris or apoptosis?

Hi all, I've recently been culturing primary rat schwann cells to prepare for experiments. The schwann cells looked fine during my previous subculture in a T-75 flask. Upon new subculturing...

09 October 2019 3,778 2 View

Does acute oxidative stress activate schwann cell proliferation?

Hi all, I am running a n experiment involving primary rat Schwann cells ( passage 3) and varying dosages of H202 (0mM, 0.1mM, 0.3mM, 0.8mM, 1mM, and 2mM). Schwann cells ( n=2,000) were grown on...

06 July 2019 2,668 5 View

Does skeletal muscle stain positive for collagen?

Hi all, I did trichrome staining for dennervated rat muscle. The results demonstrated positive aniline blue staining in the muscle at about 2 to 5% of the surface area. My question is does...

05 June 2019 582 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View