How much 100mM of Gluconic acid can change pH of a buffer?

11 December 2014 3,260 1 View

How can one decide on DNA amount, plating amount and screening size in mutant library construction?

08 September 2014 5,798 4 View

What are the most suitable conditions for E. coli DE3 sonication?

08 September 2014 246 4 View

What is the reason for my huge solvent peak?

08 September 2014 2,483 19 View

Anybody using Dynabeads?

I want to use oligoDT Dynabeads for mRNA isolation. Do you need any specific magnetic platform. Can I use the magnetic stirrer as magnet source for bead separation.

31 December 2012 3,729 4 View

What is the most important feature/parameter for considering a gene as a pseudogene?

If its reading frame has a start codon and no stop codons in between, is there any chance that it will make a protein?

07 August 2012 9,325 6 View

What is the trick to working with low volumes in a french press?

I am using constant systems French press. There is lot of volume loss and frothing happening. I am working with bacterial cell suspension and volumes are around 1-2 ml. All kind of suggestions are...

07 August 2012 9,524 5 View

Is there any tool for analyzing intergenic regions?

In order to filter out sequences on the basis of size, and stop codons.

05 June 2012 6,731 0 View

Using western blot apparatus for northern blotting?

My lab has a regular set up for protein electrophoresis and blotting. I want to use the same for RNA blotting. Which recipe is the best and least time consuming to make my whole setup RNase free?...

05 June 2012 2,155 4 View

What methods are there other than northern blotting for small RNA validation in prokaryotes?

I have some intergenic small RNA candidates in prokaryotes which I want to validate.

05 June 2012 2,571 7 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

What is the best way to fix plasma derived miRNA for subsequent cDNA synthesis?

I need to quantify blood plasma derived miRNA through q-RT-PCR. For this I need to fix RNA content in my cDNA reaction. What is the best way to do this? Currently, I take readings of my plasma...

03 February 2021 9,330 3 View

What is the general miRNA yield one gets from the Qiagen miRNEASY serum/plasma advanced kit?

I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...

02 February 2021 8,806 2 View

Which miRNA arm is a right target for analysis, 3p or 5p?

I'm working on selecting mouse miRNAs to analyze and stumbling upon one confusing issue, some of the papers do not specify which arm they analyzed and they just state something like " miR-34a was...

16 January 2021 3,792 4 View

Is negative controls necessary for western blotting?

I am trying to confirm my miRNA target by western blotting. I have a ds mimic and a ss inhibitor of my miRNA. Is it necessary to use negative controls for mimic and inhibitor? If yes, how can I...

09 January 2021 2,320 3 View

Can I predict miRNA by knowing the mRNA target?

Hello! I am trying to predict in silico the miRNA by knowing the target. Most of published research starts by knowing the miRNA and predicts the target using online tools. Are you familiar with...

31 December 2020 7,433 5 View

What are the possible drawbacks of using miRNA sponge to enhance the specificity of gene expression?

I am searching for miRNA repeats put between WPRE and 3'LTR of my lentivirus, in order to enhance the specificity of transgene expression in human neuron cells. But if clinically translation of...

26 December 2020 9,921 2 View