How much 100mM of Gluconic acid can change pH of a buffer?

11 December 2014 3,260 1 View

How can one decide on DNA amount, plating amount and screening size in mutant library construction?

08 September 2014 5,798 4 View

What are the most suitable conditions for E. coli DE3 sonication?

08 September 2014 246 4 View

What is the reason for my huge solvent peak?

08 September 2014 2,483 19 View

Anybody using Dynabeads?

I want to use oligoDT Dynabeads for mRNA isolation. Do you need any specific magnetic platform. Can I use the magnetic stirrer as magnet source for bead separation.

31 December 2012 3,729 4 View

What is the most important feature/parameter for considering a gene as a pseudogene?

If its reading frame has a start codon and no stop codons in between, is there any chance that it will make a protein?

07 August 2012 9,325 6 View

What is the trick to working with low volumes in a french press?

I am using constant systems French press. There is lot of volume loss and frothing happening. I am working with bacterial cell suspension and volumes are around 1-2 ml. All kind of suggestions are...

07 August 2012 9,524 5 View

Rule out the possibility of tRNA/rRNA in smallRNA population?

I have some smallRNA candidates. How can I confirm that they are tRNA/rRNA, any online software/program/tool available?

05 June 2012 5,923 2 View

Using western blot apparatus for northern blotting?

My lab has a regular set up for protein electrophoresis and blotting. I want to use the same for RNA blotting. Which recipe is the best and least time consuming to make my whole setup RNase free?...

05 June 2012 2,155 4 View

What methods are there other than northern blotting for small RNA validation in prokaryotes?

I have some intergenic small RNA candidates in prokaryotes which I want to validate.

05 June 2012 2,571 7 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View